Crop genomes accumulate deleterious mutations—a phenomenon known as the cost of domestication.
Precision genome editing has been proposed to eliminate such potentially harmful mutations; however, experimental demonstration is lacking. Here we identified a deleterious mutation in the tomato transcription factor SUPPRESSOR OF SP2 (SSP2), which became prevalent in the domesticated germplasm and diminished DNA binding to genome-wide targets. Researchers found that the action of SSP2 is partially redundant with that of its paralog SSP in regulating shoot and inflorescence architecture.
However, redundancy was compromised during tomato domestication and lost completely in the closely related species Physalis grisea, in which a single ortholog regulates shoot branching. Researchers applied base editing to directly repair the deleterious mutation in cultivated tomatoes and obtained plants with compact growth that provide an early fruit yield.
This research shows how deleterious variants have sensitized modern genotypes for phenotypic tuning and illustrates how repairing deleterious mutations with genome editing may allow predictable crop improvement.
Glaus, A.N., Brechet, M., Swinnen, G. et al. Repairing a deleterious domestication variant in a floral regulator gene of tomato by base editing. Nat Genet (2025). https://doi.org/10.1038/s41588-024-02026-9
Source: Nature