How tomato plants defend themselves against a devastating ‘young’ Southern African virus has now been investigated at a molecular genetics level for the first time by researchers at the University of Johannesburg (UJ).
The Ty-1 gene is known to confer resistance to the well-known tomato yellow leaf curl virus (TYLCV). UJ researchers investigated what happens when tomato plants that harbor the Ty-1 gene are infected with the relatively unknown tomato curly stunt virus (ToCSV). They found a link between tolerance to ToCSV, a plant defense called viral DNA methylation, and Ty-1 gene activity.
The research is published in Frontiers in Plant Science.
To find out more about how that happens, they used lab techniques called bisulfite conversion and PCR amplification, coupled with a next-generation whole genome virus sequencing approach.
Tomato curly stunt virus originated in South Africa about 30 years ago and is spread by whitefly. The virus can destroy an entire tomato crop, especially if enough plants are infected at a young stage. It has been found in the neighbouring country Mozambique also.
In the ongoing battle between plants and viruses, plants ‘have discovered’ that it is very effective to focus their counter-attack on the DNA inside viruses.
Read more at eurekalert.org